Selective Enriched and Differential Media

Selective Enriched and Differential Media

1 Selective, Enriched, and Differential Media Eosin Methylene Blue (p. 103 Lab Manual), MacConkey Agar (p. 109 Lab Manual), Mannitol Salt Agar (p. 97 Lab Manual) EMB (7 points) A B C The above image depicts the growth of three organisms on Eosin Methylene Blue Agar. Using the image above, please fill out the table with the results. Organism Organism A Color of Growth Interpretation of Results (heavy fermenter, lactose fermenter, non-fermenter) 2 Organism Color of Growth Organism B Organism C 1. What makes this media selective and differential? 2. What types of organisms can grow on this media? Interpretation of Results (heavy fermenter, lactose fermenter, non-fermenter) 3 MAC (7 points) The above image depicts the growth of four organisms on MacConkey Agar. Using the image above, please fill out the table with the results. Organism Organism 1 Organism 2 Organism 3 Organism 4 Color of Growth Interpretation of Results (fermenter: coliform, non coliform, Gram positive) 4 1. What types of organisms can be identified using this medium? 2. What is the pH indicator in the medium? 3. How is it selective and differential? 5 MSA (7 points) Using the image above, complete the results table. Organism Organism A Organism B Organism C Color of Growth if Present Interpretation of Results (mannitol fermenter, nonmannitol fermenter) 6 1. How is the medium selective and how is it differential? 2. What types of bacteria will grow on this medium?